Autor(es): Saengwiman, Suchada; Aroonkesorn, Aratee; Dedvisitsakul, Plaipol; Sakdee, Somsri; Leetachewa, Somphob; Angsuthanasombat, Chanan; Pootanakit, Kusol

Resumo: Bacillus thuringiensis Cry4Ba toxin selectively kills Aedes aegypti mosquito larvae as it is in part due to the presence of specific membrane-bound protein receptors. In this study, using data mining approach, we initially identified three potential glycosylphosphatidylinositol-linked aminopeptidase N (GPI-APN) isoforms, APN2778, APN2783 and APN5808, which are believed to act as Cry4Ba toxin receptors. These three isoforms that are functionally expressed in the larval midgut can be sequence-specific knocked down (ranging from 80% to 95%) by soaking the Aedes aegypti larvae in buffer of long double-stranded GPI-APN RNAs (300-680bp). Finally, to see the physiological effect of APN knockdowns, the larvae were fed with Escherichia coli expressing Cry4Ba toxin. The results revealed that all the three identified GPI-APN isoforms may possibly function as a Cry4Ba receptor, particularly for APN2783 as those larvae with this transcript knockdown showed a dramatic increase in resistance to Cry4Ba toxicity.

Palavras-Chave: Biological control; RNA; Toxicants; Receptors; Pest control; Toxicity; Toxins; Aedes aegypti; Bacillus thuringiensis; Escherichia coli

Imprenta: Biochemical and Biophysical Research Communications, v. 407, n. 4, p. 708-713, 2011.

Descritores: Aedes aegypti - Proteins ; Aedes aegypti - RNA

Data de publicação: 2011

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