Use of nitrocellulose membrane to activate and measure insect prophenol oxidase.
Autor(es): Brey, P. T.; Stoltz, D. B.; Cook, D. I.; Ashida, M.
Resumo: A method for the activation and measurement of insect (Aedes aegypti ) prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.
Palavras-Chave: Aedes aegypti; Culicidae; Diptera; Activation; Detection; Enzymes; Measurement; Measuring techniques; Membranes; Methodology; Nitrocellulose; Prophenol oxidase
Imprenta: Analytical Biochemistry, v. 194, n. 2, p. 359-364, 1991.
Descritores: Aedes aegypti - Proteins
Data de publicação: 1991
