Mortalidade e alterações morfológicas provocadas pela ação inibidora do diflubenzuron na ecdise de larvas de aedes aegypti (Diptera, Culicidae)
Autor(es): Borges, Rosana Alves; Cavasin, Glaucia Maria; da Silva, Ionizete Garcia; Arruda, Walquiria; Synthia, Ellen; de Oliveira, Fernandes; da Silva, Heloisa Helena Garcia; Martins, Flavia
Resumo: The mortality and the effect of the growth inhibitor diflubenzuron as a possible agent of structural alterations of the cuticle and midgut cells of Aedes aegypti (3rd instar larvae) was studied by light microscopy. The following dosages were compared for their ability to induce alterations on cuticle and midgut of the larvae: minimal (0,1 ppm), and maximum (1 ppm) lethal dosage. The main alterations observed at light microscopy were the presence of cytoplasmatic vacuoles and increased concentration of secretion on apical surface of the midgut cells. Moreover, the cuticle thickness, with great amount of nucleus, provided evidence of the high cellular activity. Also, the fat body of the larvae showed alterations. Investigations demonstrated that the midgut epithelial cells presented highly vacuolated, spongious, and with secretory vesicles of several sizes. Observations showed aspects of the larval change process, as evidenced by formation of subcuticular space, which is a characteristic of the initial change stage, and by presence of epicuticle and exocuticle separated by an exuvial space.
Imprenta: Revista de Patologia Tropical, v. 33, n. 1, p. 91-104, 2004
Identificador do objeto digital: 10.5216/rpt.v33i1.3248
Descritores: Aedes aegypti - Larvicide
Data de publicação: 2004
