Molecular characterization and enzymatic analysis of juvenile hormone epoxide hydrolase genes in the red flour beetle Tribolium castaneum
Autor(es): Tsubota, T.; Nakakura, T.; Shiotsuki, T.
Resumo: Juvenile hormone epoxide hydrolases (JHEHs) degrade juvenile hormones (JHs) and are important for JH titre regulation. Here, we report the cloning and analysis of five jheh-related (jheh-r1-r5) genes in the red flour beetle, Tribolium castaneum, a model species for the coleopteran insects. T. castaneum JHEH-r (TcJHEH-r) proteins show high homology to lepidopteran JHEHs and also to human microsomal epoxide hydrolase. In the phylogenetic tree, Tcjheh-rs were clustered, and interestingly, they were also clustered in the genome. Examination of enzymatic activities using recombinant TcJHEH-r proteins showed that TcJHEH-r3 had strong degradation activity for JH III, whereas TcJHEH-r4 had weak activity. The study has yielded significant information that will facilitate further analysis of JHEHs and epoxide hydrolases.
Palavras-Chave: JHEH; Cdna Cloning; Epoxide Hydrolase; Detoxification Enzyme; Coleoptera
Imprenta: Insect Molecular Biology, v. 19, n. 3, p. 399-408, 2010
Identificador do objeto digital: 10.1111/j.1365-2583.2010.01001.x
Descritores: Aedes aegypti - Genome
Data de publicação: 2010
