Molecular analysis of the serine/threonine kinase Akt and its expression in the mosquito Aedes aegypti
Autor(es): Riehle, MA; Brown, MR
Resumo: A key component of the insulin-signalling pathway, the protein kinase Akt, was identified and cloned as a cDNA from ovaries of the mosquito Aedes aegypti . An ortholog gene was found in the Anopheles gambiae genome database, and like other Akts, both mosquito Akts possess pleckstrin homology domains for membrane binding and a serine/threonine kinase domain. When Ae. aegypti ovaries were treated with bovine insulin in vitro , a putative Akt was threonine-phosphorylated, as expected for Akts. AaegAKT was only expressed in embryos for the first 6 h after oviposition and in ovaries before and during a gonotrophic cycle.
Palavras-Chave: Protein Kinase B (PKB); Insect; Ecdysteroids; Oogenesis; Ovary
Imprenta: Insect Molecular Biology, v. 12, n. 3, p. 225-232, 2003
Identificador do objeto digital: 10.1046/j.1365-2583.2003.00405.x
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome
Data de publicação: 2003
