Mitochondrial cytochrome oxidase i gene sequence analysis of Aedes albopictus in Malaysia
Autor(es): Ismail, Nurul-Ain; Dom, Nazri Che; Ismail, Rodziah; Ahmad, Abu Hassan; Zaki, Afiq; Camalxaman, Siti Nazrina
Resumo: A study was conducted to establish polymorphic variation of the mitochondrial DNA encoding the cytochrome oxidase subunit 1 (CO1) gene in Aedes albopictus isolated from 2 hot spot dengue-infested Areas in the Subang Jaya District, Malaysia. A phylogenetic analysis was performed with the use of Sequences obtained from USJ6 and Taman Subang Mas (TSM). Comparison of the local CO1 Sequences with a laboratory strain (USM), alongside reference strains derived from the GenBank database revealed low genetic variation in terms of nucleotide differences and haplotype diversity. Four methods were used to construct a phylogenetic tree and illustrate the genetic relationship of the 37 Ae. albopictus populations based on the CO1 Sequences, namely neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML), and Bayesian method, which revealed a distinct relationship between isolates from USJ6 and TSM. Our findings provide new information regarding the genetic diversity among morphologically similar Ae. albopictus, which has not been reported to date.
Palavras-Chave: Aedes Albopictus; Haplotype; Mitochondrial DNA Cytochrome Oxidase 1; Polymorphism; Sequence Variation
Imprenta: Journal of the American Mosquito Control Association, v. 31, n. 4, p. 305-312, 2015
Descritores: Aedes aegypti - DNA
Data de publicação: 2015
