Maximal toxicity of cloned CytA delta-endotoxin from Bacillus thuringiensis subsp israelensis requires proteolytic processing from both the N- and C-termini
Autor(es): Alyahyaee, SAS; Ellar, DJ
Resumo: Unlike the CytA toxin from native Bacillus thuringiensis subsp. israelensis (Bti) crystals, the inclusions of cloned CytA produced by Bti IPS 78/11 in the presence of the 20 kDa 'helper' protein require a reducing agent in addition to a highly alkaline ph for complete solubilization. Activation of the solubilized CytA with a range of proteases produced 25-22 kDa products. SDS-PACE analysis and N-terminal amino acid sequencing revealed that CytA was processed very similarly at both termini by proteinase K or by Anopheles or Culex gut extracts, Trypsin, by contrast, processed CytA predominantly at the N-terminus, In vitro cytolytic assays against Aedes aegypti cells, and haemolytic assays against rat erythrocytes, showed that CytA processed at both termini by proteinase K was the most active form. Thus CytA, like other Bt delta-endotoxins, is processed to a well-defined protease-resistant product and this processing enhances the toxicity in vitro and possibly in vivo.
Palavras-Chave: Bacillus Thuringiensis; Delta-Endotoxins; Cyta; Haemolytic Assay
Imprenta: Microbiology-UK, v. 141, p. 3141-3148, 1995
Descritores: Aedes aegypti - Cell
Data de publicação: 1995
