ISWpi1 from Wolbachia pipientis defines a novel group of insertion Sequences within the IS5 family
Autor(es): Cordaux, Richard
Resumo: Insertion Sequences are transposable elements that can represent substantial proportions of prokaryotic genomes and play a substantial role in shaping host genome Evolution. As such, evaluating and understanding insertion Sequence diversity is an important task to fulfill, because it is expected to yield new insight into the Evolution of bacterial transposable elements and contribute to improve genome annotations. Here, I characterized an insertion Sequence, termed ISWpil, for which the taxonomic distribution appears to be restricted to the obligate intracellular alpha-Proteobacterium Wolbachia pipientis. ISWpil exhibits similar to 46% identity at the amino acid level with members of the IS1031 group of insertion Sequences from the IS5 family. However, the IS1031 group is characterized by a transposase gene encoded by a single open reading frame, whereas the ISWpil transposase gene consists of two overlapping open reading frames presumably translated as a single protein via programmed translational frameshifting. Such structure suggests that ISWpil may instead be related to the IS427 group of insertion Sequences from the IS5 family. Altogether, these data indicate that ISWpil extends the known spectrum of diversity of the IS5 family, and I propose to define a novel group of insertion Sequences within the IS5 family typified by ISWpil. Probable transpositional activity, relevant insertion site preferences and taxonomic specificity make ISWpil a promising tool for experimentally manipulating W pipientis bacteria, especially in light of the increasing interest in developing these bacteria as tools for controlling insect disease vectors and agricultural pests. (C) 2007 Elsevier B.V. All rights reserved.
Palavras-Chave: Transposable Element; Bacteria; Characterization; Diversity; Transposase; Programmed Translational Frameshifting
Imprenta: Gene, v. 409, n. 42401, p. 20-27, 2008
Identificador do objeto digital: 10.1016/j.gene.2007.10.035
Descritores: Aedes aegypti - Genome
Data de publicação: 2008
