Autor(es): Silva, RO; Silva, HHG; Ulhoa, CJ; Luz, C

Resumo: The larvicidal effect of Metarhizium anisopliae (Metschn.) Sorokin (Hyphomycetes) isolated in peridomestic Areas in Central Brazil was tested in Aedes aegypti (L.) (Dipt., Culicidae), which is worldwide the primary vector for the viruses that cause human dengue and yellow fever, Highest susceptibility of larvae was found after application of suspended ungerminated conidia. However, conidia, which were found on the larval Cuticle and in the gut did not germinate in live or dead larvae. Mortality dropped when testing germinating conidia or supernatants, which originated from chitin-amended minimal medium (MM) inoculated conidia and cultures up to 72 h. Paralysis of larval movement was observed a few hours after application, especially Of untreated conidia, isolates showed a high variability of total protein production and N-acetyl-beta-D-glucosaminidase activity after 4,K and 72 h incubation in MM. No relationship between enzyme levels and insecticidal activity Could be detected. The results indicate that toxic compounds emitted by ungerminated conidia on the cuticle or in the gut are involved in the activity of. M. anisopliae against A. aegypti larvae.

Palavras-Chave: Brazil; Chitinase; Entomopathogenic Fungi; Larvicidal Activity; Mosquito; Vector Control

Imprenta: Journal of Applied Entomology, v. 129, n. 3, p. 158-164, 2005

Identificador do objeto digital: 10.1111/j.1439-0418.2005.00933.158-164

Descritores: Aedes aegypti - Larvicide ; Aedes aegypti - Public health

Data de publicação: 2005

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