Integration of the Aedes aegypti mosquito genetic linkage and physical maps
Autor(es): Brown, SE; Severson, DW; Smith, LA; Knudson, DL
Resumo: Two approaches were used to correlate the Aedes aegypti genetic linkage map to the physical map. STS markers were developed for previously mapped RFLP-based genetic markers so that large genomic clones from cosmid libraries could be found and placed to the metaphase chromosome physical maps using standard FISH methods. Eight cosmids were identified that contained eight RFLP marker Sequences, and these cosmids were located on the metaphase chromosomes. Twenty-one cDNAs were mapped directly to metaphase chromosomes using a FISH amplification procedure. The chromosome numbering schemes of the genetic linkage and physical maps corresponded directly and the orientations of the genetic linkage maps for chromosomes 2 and 3 were inverted relative to the physical maps. While the chromosome 2 linkage map represented essentially 100% of chromosome 2, similar to 65% of the chromosome 1 linkage map mapped to only 36% of the short p-arm and 83% of the chromosome 3 physical map contained the complete genetic linkage map. Since the genetic linkage map is a RFLP cDNA-based map, these data also provide a minimal estimate for the size of the euchromatic regions. The implications of these findings on positional cloning in A. aegypti are discussed.
Palavras-Chave: Anopheles-Gambiae; Human-Chromosomes; Vector Mosquito; Malaria Vector; Susceptibility; Landmarks; Genome; Fish; Loci; Polymorphisms
Imprenta: Genetics, v. 157, n. 3, p. 1299-1305, 2001
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome
Data de publicação: 2001
