Insulin-Related Peptide 5 is Involved in Regulating Embryo Development and Biochemical Composition in Pea Aphid with Wing Polyphenism
Autor(es): Guo, Shan-Shan; Zhang, Meng; Liu, Tong-Xian
Resumo: In aphids there is a fecundity-dispersal trade-off between wingless and winged morphs. Recent research on the molecular mechanism of wing morphs associated with dispersal reveals that insulin receptors in the insulin signaling (IS) pathway regulate alternation of wing morphs in planthoppers. However, little is known about whether genes in the IS pathway are involved in developmental regulation in aphid nymphs with different wing morphs. In this study, we show that expression of the insulin-related peptide 5 gene (Apirp5) affects biochemical composition and embryo development of wingless pea aphids, Acyrthosiphon pisum. After comparing expression levels of major genes in the IS pathway between third instar winged and wingless nymphs, we found that Apirp5 showed higher expression in head and thorax in the wingless nymphs than in the winged nymphs. Although microinjection treatment affects physical performance in aphids, nymphs with RNA interference of Apirp5 had less weight, smaller embryos, and higher carbohydrate and protein contents compared to the control group. Comparison between winged and wingless nymphs showed a similar trend. These results indicate that Apirp5 is involved in embryo development and metabolic regulation in wing dimorphic pea aphid.
Palavras-Chave: Aphid; Insulin-Like Peptide; Wing Polyphenism; Wing Differentiation; Biochemical Components
Imprenta: Frontiers in Physiology, v. 7, 2016
Identificador do objeto digital: 10.3389/fphys.2016.00031
Descritores: Aedes aegypti - RNA
Data de publicação: 2016
