Autor(es)d'Avila-Levy, CM; Dias, FCD; de Melo, ACN; Martins, JL; Lopes, AHDC; Dos Santos, ALS; Vermelho, AB; Branquinha, MH

ResumoAny actual understanding of trypanosomatids in general requires a comprehensive analysis of the less-specialized species as thorough as our knowledge of the more specialized Leishmania and Trpanosoma. In this context, we have shown by antibody cross-reactivity that purified extracellular metallopeptidases from Phytomonas francai, Crithidia deanei (cured strain) and Crithialia guilhermei share common epitopes with the leishmanial gp63. Flow cytometry and fluorescence microscopy analyses indicated the presence of gp63-like molecules on the cell surface of these lower trypanosomatids. Binding assays with explanted guts of Aedes aegypti incubated with purified gp63 and the pretreatment of trypanosomatids with anti-gp63 antibodies indicated that the gp63-like molecules are involved in the adhesive process of these trypanosomatids to the A. aegypti gut wall. In addition, our results indicate for the first time that the gp63-like molecule binds to a polypeptide of 50 kDa. on the A. aegypti gut epithelium extract.

Palavras-ChaveTrypanosomatids; Gp63; Cellular Interaction; Aedes Aegypti; Phytomonas; Crithidia

ImprentaFems Microbiology Letters, v. 254, n. 1, p. 149-156, 2006

DescritoresAedes aegypti - Biochemistry ; Aedes aegypti - Cell

Data de Publicação:2006