Insect-Specific Flaviviruses from Culex Mosquitoes in Colorado, with Evidence of Vertical Transmission
Autor(es): Bolling, Bethany G.; Eisen, Lars; Moore, Chester G.; Blair, Carol D.
Resumo: Mosquitoes were collected in Colorado during 2006 and 2007 to examine spatial and seasonal patterns of risk for exposure to Culex vectors and West Nile virus. We used universal flavivirus primers to test pools of Culex mosquitoes for viral RNA. This led to the detection and subsequent isolation of two insect-specific flaviviruses: Culex flavivirus (CxFV), which was first described from Japan, and a novel insect flavivirus, designated Calbertado virus (CLBOV), which has also been detected in California and Canada. We recorded both viruses in Cx. tarsalis and Cx. pipiens from Colorado. Furthermore, quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed the presence of CxFV RNA in Cx. pipiens eggs and larvae from a laboratory colony established in 2005 and naturally infected with CxFV, suggesting vertical transmission as a means of viral maintenance in natural Culex populations. Finally, we present phylogenetic analyses of the relationships between insect-specific flaviviruses and other selected flaviviruses.
Palavras-Chave: West-Nile-Virus; Fusing Agent Virus; Yellow-Fever Virus; Aedes-Aegypti; Encephalitis-Virus; Pcr Assay; Vector; Population; Albopictus; Sequences
Imprenta: American Journal of Tropical Medicine And Hygiene, v. 85, n. 1, p. 169-177, 2011
Identificador do objeto digital: 10.4269/ajtmh.2011.10-0474
Descritores: Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR
Data de publicação: 2011
