Autor(es): d'Avila-Levy, CM; Silva, BA; Hayashi, EA; Vermelho, AB; Alviano, CS; Saraiva, EMB; Branquinha, MH; Santos, ALS

Resumo: Blastocrithidia culicis and Crithidia deanei are trypanosomatid protozoa of insects that normally contain intracellular symbiotic bacteria. The protozoa can be rid of their endosymbionts by antibiotics, producing a cured cell line. Here, we analyzed the glycoconjugate profiles of endosymbiont-harboring and cured strains of B. culicis and C. deanei by Western blotting and flow cytometry analyses using lectins that recognize specifically sialic acid and mannose-like residues. The absence of the endosymbiont increased the intensity of the lectins binding on both trypanosomatids. In addition, wild and cured strain-specific glycoconjugate bands were identified. The role of the surface saccharide residues on the interaction with explanted guts from Aedes aegypti gut was assessed. The aposymbiotic strains of B. culicis and C. deanei presented interaction rates 3.3- and 2.3-fold lower with the insect gut, respectively, when compared with the endosymbiont-bearing strains. The interaction rate of sialidase-treated cells of the wild and cured strains of B. culicis and C deanei was reduced in at least 90% in relation to the control. The interaction of B. culicis (wild strain) with explanted guts was inhibited in the presence of mucin (56%), fetuin (62%), sialyllactose (64%) and alpha-methyl-(D)-mannoside (80%), while in C. deanei (wild strain) the inhibition was 53%, 56%, 79% and 34%, respectively. Collectively, our results suggest a possible involvement of sialomolecules and mannose-rich glycoconjugates in the interaction between insect trypanosomatids and the invertebrate host. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V.All rights reserved.

Palavras-Chave: Crithidia; Blastocrithidia; Sialic Acid; Glycoconjugates; Cellular Interaction; Endosymbiont; Lectin; Aedes Aegypti

Imprenta: Fems Microbiology Letters, v. 252, n. 2, p. 279-286, 2005

Identificador do objeto digital: 10.1016/j.femsle.2005.09.012

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell

Data de publicação: 2005

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