Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti
Autor(es): Shao, L; Devenport, M; Fujioka, H; Ghosh, A; Jacobs-Lorena, M
Resumo: Immuno-screening of an adult Aedes aegypti midgut cDNA expression library with anti-peritrophic matrix antibodies identified cDNAs encoding a novel peritrophic matrix protein, termed Ae. aegypii Adult Peritrophin 50 (Ac-Aper50), and the epithelial cell-surface membrane protein, AEG12. Both genes are expressed exclusively in the midguts of adult female mosquitoes and their expression is strongly induced by blood Feeding. Ae-Aper50 has a predicted secretory signal peptide and five chitin-binding domains with intervening mucin-like domains. Localization of Ae-Aper50 to the peritrophic matrix was demonstrated by immuno-electron microscopy. Recombinant Ae-Aper50 expressed in baculovirus-infected insect cells binds chitin in vitro. Site-directed mutagenesis was used to study the role that cysteine residues from a single chitin-binding domain play in the binding to a chitin substrate. Most of the cysteine residues proved to be critical for binding. AEG12 has a putative secretory signal peptide at the amino-terminus and a putative glycosyl-phosphatidylinositol (GPI) anchor signal at its carboxyl-terminus and the protein was localized by immuno-electron microscopy to the midgut epithelial cell microvilli. (C) 2005 Elsevier Ltd. All rights reserved.
Palavras-Chave: Mosquito; Aedes Aegypti; Midgut; Microvili; Peritrophic Matrix
Imprenta: Insect Biochemistry and Molecular Biology, v. 35, n. 9, p. 947-959, 2005
Identificador do objeto digital: 10.1016/j.ibmb.2005.03.012
Descritores: Aedes aegypti - Molecular screening
Data de publicação: 2005
