Autor(es): Dixit, R; Dixit, S; Gakhar, S

Resumo: Lysozyme (E.C. 3.2.1.17) activity is reported from the malaria vector Anopheles slephensi. The activity was detected in the salivary gland and midgut using bacteriolytic radial diffusion assay. Spectrophotometric analysis indicated that higher level of lysozyme activity was maintained in both midgut and salivary gland tissues. The activity reached the highest level in 4-8 days old mosquitoes. Genomic PCR amplification revealed the presence of at least two putative lysozyme genes in the mosquito genome. Preliminary analysis of one of the 413 bp genomic fragments showed 56% identity to the lysozyme of mosquito A. gambiae. However, the nature and origin of the putative cloned lysozyme gene remains elusive.

Palavras-Chave: Lysozyme; Mosquito; Malaria; Salivary Gland; Midgut; Anopheles Stephensi

Imprenta: Indian Journal of Biochemistry & Biophysics, v. 43, n. 1, p. 15-19, 2006

Descritores: Aedes aegypti - Genome

Data de Publicação: 2006