Gene expression in Plasmodium berghei ookinetes and early oocysts in a co-culture system with mosquito cells
Autor(es): Vontas, J; Siden-Kiamos, I; Papagiannakis, G; Karras, M; Waters, AP; Louis, C
Resumo: Using an in vitro development system for Plasmodium berghei sporogonic stages and microarray technology we examined parasite gene expression during ookinete invasion of Aedes cells and the ensuing oocyst development. A number of genes were found to be differentially expressed. The most prominent class of up-regulated elements corresponded to products involved in protein synthesis and metabolism. Furthermore, several previously studied genes with a known in vivo developmental profile matched published data. A large number of genes with a hitherto unknown function during the life cycle stages studied also show a differential pattern of expression, indicating the involvement of their products in control and execution of active developmental processes. (C) 2004 Elsevier B.V. All rights reserved.
Palavras-Chave: Aedes Aegypti; Malaria; Microarrays; Genomics
Imprenta: Molecular and Biochemical Parasitology, v. 139, n. 1, p. 1-13, 2005
Identificador do objeto digital: 10.1016/j.molbiopara.2004.03.003
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - Protein synthesis ; Aedes aegypti - Proteins
Data de publicação: 2005
