Autor(es): Souza, Terezinha M.; Bezerra Menezes, Erika S.; Oliveira, Rodrigo V.; Filho, Luiz Carlos P. Almeida; Martins, Jorge M.; Moreno, Frederico B.; Monteiro-Moreira, Ana Cristina O.; Araripe Moura, Arlindo A.; Urano Carvalho, Ana F.

Resumo: Nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector Aedes aegypti. Besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. Thus, the present work aimed to assess changes in protein expression of 3rd-instar larvae of Ae. aegypti after exposure to the natural insecticide m-pentadecadienyl-phenol. Bidimensional electrophoresis followed by mass spectrometry resulted in identification of 12 proteins differentially expressed between control and treated groups. Larvae exposed to the toxic compound for 24 h showed elevated detoxification response (glutathione-S-transferase), increased levels of stress-related proteins (HSP70) as well as evidence of lysosome stabilization to enable survival. Furthermore, expression of proteins involved in protection of peritrophic membrane and metabolism of lipids indicated systemic effect of toxic effects in treated larvae. (C) 2015 Elsevier B.V. All rights reserved.

Palavras-Chave: Comparative proteomics; Detoxification; Natural insecticide; m-Pentadecadienyl-phenol

Imprenta: Acta Tropica, v. 152, p. 49-55, 2015

Identificador do Objeto Digital: 10.1016/j.actatropica.2015.08.010

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Proteome ; Aedes aegypti - Larvicide ; Aedes aegypti - Public health

Data de Publicação: 2015