First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera : Culicidae): new perspectives for an insect-bacteria association
Autor(es): Gusmao, Desiely Silva; Santos, Adao Valmir; Marini, Danyelle Cristine; Russo, Erica de Souza; Dias Peixoto, Anelise Maria; Bacci, Mauricio, Jr.; Berbert-Molina, Marilia Amorim; Alves Lemos, Francisco Jose
Resumo: We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
Palavras-Chave: Aedes Aegypti; Gut Diverticulum; Microbiota; Serratia Sp.
Imprenta: Memorias do Instituto Oswaldo Cruz, v. 102, n. 8, p. 919-924, 2007
Identificador do objeto digital: 10.1590/S0074-02762007000800005
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2007
