First isolation of an Entomobirnavirus from free-living insects
Autor(es): Marklewitz, Marco; Gloza-Rausch, Florian; Kurth, Andreas; Kuemmerer, Beate Mareike; Drosten, Christian; Junglen, Sandra
Resumo: Drosophila X virus (DXV), the prototype Entomobirnavirus, is a well-studied RNA virus model. Its origin is unknown, and so is that of the only other entomobirnavirus, Espirito Santo virus (ESV). We isolated an entomobirnavirus tentatively named Culex Y virus (CYV) from hibernating Culex pipiens complex mosquitoes in Germany. CYV was detected in three pools consisting of 11 mosquitoes each. Full-genome sequencing and phylogenetic analyses suggested that CYV and ESV define one sister species to DXV within the genus Entomobirnavirus. In contrast to the laboratory-derived ESV, the ORF5 initiation codon AUG was mutated to (1927)GUG in all three wild-type CYV isolates. Also in contrast to ESV, replication of CYV was not dependent on other viruses in insect cell culture. CYV could provide a wild-type counterpart in research fields relying on DXV and other cell culture-adapted strains.
Palavras-Chave: Drosophila-X-Virus; Bursal Disease Virus; Rna Interference; Dengue Virus; Electron Cryomicroscopy;Sequence-Analysis; Initiation Codon; Infected-Cells; Aedes-Aegypti; B2 Protein
Imprenta: Journal of General Virology, v. 93, n. 11, p. 2431-2435, 2012
Identificador do objeto digital: 10.1099/vir.0.045435-0
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - RNA ; Aedes aegypti - Virus
Data de publicação: 2012
