Autor(es): Chotkowski, Heather L.; Ciota, Alexander T.; Jia, Yongqing; Puig-Basagoiti, Francesc; Kramer, Laura D.; Shi, Pei-Yong; Glaser, Robert L.

Resumo: To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of similar to 25 nt WNV-homologous small RNAs was detected in infected S2 cells but not C6/36 cells. RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous small RNAs produced in infected S2 cells are functional small interfering RNAs. WNV was shown to infect adult D. melanogaster, and adult flies containing mutations in each of four different RNAi genes (Argonaute 2, spindle-E, piwi, and Dicer-2) were significantly more susceptible to WNV infection than wildtype flies. These results combined with the analysis of WNV infection of S2 and C6/36 cells support the conclusion that WNV infection of D. melanogaster, but perhaps not Ae. albopictus, induces a protective RNAi response. (c) 2008 Elsevier Inc. All rights reserved.

Palavras-Chave: RNAi; Innate immunity; West Nile virus; Drosophila

Imprenta: Virology, v. 377, n. 1, p. 197-206, 2008

Identificador do objeto digital: 10.1016/j.virol.2008.04.021

Descritores: Aedes aegypti - Cell ; Aedes aegypti - RNA ; Aedes aegypti - Virus

Data de publicação: 2008

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