Viewpoint: High susceptibility to Chikungunya virus of Aedes aegypti from the French West Indies and French Guiana
Autor(es): Girod, Romain; Gaborit, Pascal; Marrama, Laurence; Etienne, Manuel; Ramdini, Cedric; Rakotoarivony, Ignace; Dollin, Christelle; Carinci, Romuald; Issaly, Jean; Dusfour, Isabelle; Gustave, Joel; Yp-Tcha, Marie-Michelle; Yebakima, Andre; Failloux, Anna-Bella; Vazeille, Marie
Resumo: To estimate the vector competence of Aedes aegypti populations sampled from distinct anthropogenic environments in French Guiana, Guadeloupe and Martinique for the strain CHIKV 06.21.MethodsF-1/F-2 females were orally infected at titres of 106 and 107.5 pfu/ml in blood-meals. Disseminated infection rates (DIR) of mosquitoes were estimated using indirect fluorescent antibody assay on heads' squashes, 7 or 14 days post-infection (pi).ResultsAt a titre of 107.5 pfu/ml, DIR ranged from 88.9% to 100.0% and were not significantly different whether assessed at day 7 or 14 pi. At a titre of 106 pfu/ml, DIR observed 7 days pi ranged from 37.6 to 62.0%.ConclusionsAe. aegypti from French Guiana and French West Indies are highly competent to transmit CHIKV. An evaluation of DIR 7 days rather than 14 days pi is adequate to estimate vector competence. The titre of 106 pfu/ml allows us to distinguish Ae. aegypti populations originating from distinct environments (dense or diffuse housing) by their vector competence. This assessment is a prerequisite to better evaluate the potential risk of Chikungunya outbreaks once the virus is introduced from endemic regions.
Palavras-Chave: Aedes aegypti; Chikungunya virus; Oral infections; Vector competence; French West Indies; French Guiana
Imprenta: Tropical Medicine & International Health, v. 16, n. 1, p. 134-139, 2011
Identificador do objeto digital: 10.1111/j.1365-3156.2010.02613.x
Descritores: Aedes aegypti - Chikungunya Fever ; Aedes aegypti - Epidemiology ; Aedes aegypti - Public health
Data de publicação: 2011
