Autor(es): Das, Suchismita; Radtke, Andrea; Choi, Young-Jun; Mendes, Antonio M.; Valenzuela, Jesus G.; Dimopoulos, George

Resumo: The Anopheles gambiae salivary glands play a major role in malaria transmission and express a variety of bioactive components that facilitate blood-feeding by preventing platelet aggregation, blood clotting, vasodilatation, and inflammatory and other reactions at the probing site on the vertebrate host.Results: We have performed a global transcriptome analysis of the A. gambiae salivary gland response to blood-feeding, to identify candidate genes that are involved in hematophagy. A total of 4,978 genes were found to be transcribed in this tissue. A comparison of salivary gland transcriptomes prior to and after blood-feeding identified 52 and 41 transcripts that were significantly up-regulated and down-regulated, respectively. Ten genes were further selected to assess their role in the blood-feeding process using RNAi-mediated gene silencing methodology. Depletion of the salivary gland genes encoding D7L2, anophelin, peroxidase, the SG2 precursor, and a 5'nucleotidase gene significantly increased probing time of A. gambiae mosquitoes and thereby their capacity to blood-feed.Conclusions: The salivary gland transcriptome comprises approximately 38% of the total mosquito transcriptome and a small proportion of it is dynamically changing already at two hours in response to blood feeding. A better understanding of the salivary gland transcriptome and its function can contribute to the development of pathogen transmission control strategies and the identification of medically relevant bioactive compounds.

Palavras-Chave: Adult female mosquito; Plasmodium invasion; Binding protein; Immune system; Family; Genes

Imprenta: BMC Genomics, v. 11, 2010

Identificador do objeto digital: 10.1186/1471-2164-11-566

Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure

Data de publicação: 2010

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