Transcriptional control is relevant in the modulation of mosquito ferritin synthesis by iron
Autor(es): Pham, D. Q. D.; Winzerling, J. J.; Dodson, M. S.; Law, J. H.
Resumo: In yellow fever mosquito cells (Aag2 clone), iron treatment induces a threefold increase in ferritin message (fer mRNA) and protein (ferritin) by 16 h. These data contrast with work in mammalian hepatocytes and fibroblasts in which Ser mRNA levels do not change with iron stimulation, but ferritin levels increase 50-fold. Pretreatment of the Aag2 cells with actinomycin D blocks induction of fer mRNA and reduces the ferritin subunit synthesis, suggesting that iron induction of ferritin subunit synthesis is subjected to transcriptional control. A putative iron-regulatory protein has also been identified in cytoplasmic extracts from Aag2 cells.
Palavras-Chave: Aedes aegypti; Ferritin; Mosquito; Transcription; Translation
Imprenta: European Journal of Biochemistryt, v. 266, n. 1, p. 236-240, 1999
Identificador do objeto digital: 10.1046/j.1432-1327.1999.00849.x
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Protein synthesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA
Data de publicação: 1999
