Autor(es)Graham, D. H.; Holmes, J. L.; Beaty, B. J.; Black, W. C.

ResumoQuantitative trait loci (QTL) affecting the ability of the Eastern treehole mosquito, Ochlerotatus triseriatus, to transovarially transmit (TOT) La Crosse virus (LAC) were mapped in an F-1 intercross. The Holmen strain of O. triseriatus, previously selected for TOT refractoriness, was crossed to the AIDL strain that had been selected for TOT permissiveness. In P-1 and F-1 parents and 49 F2 individuals, regions of 10 cDNA loci were analysed with single strand conformation polymorphism (SSCP) analysis to identify and orientate linkage groups. Genotypes were also scored at fifty-six random amplified polymorphic DNA (RAPD)-SSCP loci. Twenty-eight F-2 offspring were individually analysed for TOT. Three QTL for TOT were detected with standard interval mapping on chromosomes II and III. Alleles at the three loci contributed additively towards determining the overall TOT rate and cumulatively accounted for approximate to 53% of the phenotypic variance in TOT.

Palavras-ChaveOchlerotatus triseriatus; La Crosse virus; Transovarial transmission; Quantitative trait loci

ImprentaInsect Molecular Biology, v. 12, n. 4, p. 307-318, 2003

DescritoresAedes aegypti - DNA

Data de Publicação:2003