Autor(es): Anderson, J. R.; Schneider, J. R.; Grimstad, P. R.; Severson, D. W.

Resumo: La Crosse virus is a leading cause of pediatric encephalitis in the United States. The mosquito Ochterolatus triseriatus is an efficient vector for La Crosse virus, whereas the closely related O. hendersoni transmits only at very low rates. Quantitative trait loci (QTL) affecting the ability to orally transmit this virus and adult body size were identified in 164 F, female individuals from interspecific crosses of O. hendersoni females and O. triseriatus males rising a combination of composite interval mapping (CIM), interval mapping (IM) for binary traits, and single-marker mapping. For oral transmission (OT), no genome locations exceeded the 95% experimentwise threshold for declaring a QTL using IM, but single-marker analysis identified four independent regions significantly associated with OT that we considered as tentative QTL. With two QTL, an increase in OT was associated with alleles from the refractory vector, O. hendersoni, and likely reflect epistatic interactions between genes that were uncovered by our interspecific crosses. For body size, two QTL were identified using CIM and a third tentative QTL was identified using single-marker analysis. The genome regions associated with body size also contain three QTL controlling OT, suggesting that these regions contain either single genes with pleiotropic effects or multiple linked genes independently determining each trait.

Palavras-Chave: Western equine encephalomyelitis; Mosquito Aedes aegypti; Trait loci; Lacrosse virus; Anopheles-gambiae; Transovarial transmission; Extrinsic incubation; Salivary glands; Dengue 2 virus; Culex tarsalis

Imprenta: Genetics, v. 169, n. 3, p. 1529-1539, 2005

Identificador do objeto digital: 10.1534/genctics.104.01639

Descritores: Aedes aegypti - Genome

Data de publicação: 2005