Quantitative analysis of replication and tropisms of dengue virus type 2 in Aedes albopictus
Autor(es): Zhang, Meichun; Zheng, Xiaoying; Wu, Yu; Gan, Ming; He, Ai; Li, Zhuoya; Liu, Jing; Zhan, Ximei
Resumo: Dengue virus serotype 2 (DENV-2) RNA replication profiles and tropisms were studied by using quantitative RT-PCR (q-RTPCR) in intrathoracically infected Aedes albopictus The virus RNA replication profiles were diverse in mosquito organs In fat body, brain, salivary gland. and malpighian tubes. it peaked at 8,23, 23, and 27 days post-infection. respectively. and then, all declined In midgut, it increased all the time and had no trend of decline. In ovary, it had no apparent Increase Subsequent Western blotting of DENV-2 E protein had similar results Using ribosomal protein 7 (rpS7) as an internal control, we found that, in salivary gland. brain, fat body, and midgut. the average DENV-2 RNA levels DENV-2 RNA/rpS7 mRNA) were 1.028, 464,5 6. and 6 2, respectively, in malpighian tubes, it was I. and in ovary, it was far less than 1 These results suggest that infection profiles and tropism of DENV-2 RNA in Ae albopictus organs are significantly different
Palavras-Chave: Trans-ovarial transmission; Vector competence; Geographic strains; Aegypti mosquitos; Culicidae; Diptera; Susceptibility
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 83, n. 3, p. 700-707, 2010
Identificador do objeto digital: 10.4269/ajtmh.2010.10-0193
Descritores: Aedes aegypti - RT-PCR
Data de publicação: 2010
