Autor(es): Badisco, Liesbeth; Claeys, Ilse; Van Hiel, Matthias; Clynen, Elke; Huybrechts, Jurgen; Vandersmissen, Tim; Van Soest, Sofie; Bosch, Luc Vanden; Simonet, Gert; Broeck, Jozef Vanden

Resumo: Members of the insulin superfamily are not restricted to vertebrates, but have also been identified in invertebrate species. In the current report, we present the characterization of Scg-insulin-related peptide (IRP), an insulin-related peptide in the desert locust, Schistocerca gregaria. This peptide was isolated from corpora cardiaca (CC) extracts by means of a high-performance liquid chromatography (HPLC)-based purification strategy. Subsequent cloning and sequencing of the corresponding cDNA revealed that the encoded Scg-IRP precursor displays the structural organization that is typical for members of the insulin superfamily. Moreover, immunocytochemistry on brain tissue sections demonstrated the presence of Scg-IRP in median neurosecretory cells of the pars intercerebralis and their projections towards the storage part of the CC. Quantitative real-time RT-PCR studies revealed the presence of Scg-IRP transcripts in a variety of tissues, including nervous tissue and fat body. Furthermore, these transcripts showed a tissue- and phase-dependent, temporal regulation during the reproductive cycle of adult males and females. Finally, we demonstrated that Scg-IRP interacts in vitro with a recombinant neuroparsin, a locust protein displaying sequence similarity with vertebrate IGF binding proteins.

Palavras-Chave: Mosquito Aedes-aegypti; Amino-acid-sequence; Corpora cardiaca; Prothoracicotropic hormone; Molecular characterization; RT-PCR; Expression; Drosophila; Cells; Identification

Imprenta: Journal of Molecular Endocrinology, v. 40, p. 137-150, 2008

Identificador do objeto digital: 10.1677/JME-07-0161

Descritores: Aedes aegypti - RT-PCR ; Aedes aegypti - Real Time PCR

Data de publicação: 2008

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