Plasmodium vivax: Impaired escape of Vk210 phenotype ookinetes from the midgut blood bolus of Anopheles pseudopunctipennis
Autor(es): Gonzalez-Ceron, Lilia; Rodriguez, Mario H.; Chavez-Munguia, B.; Santillan, F.; Nettel, Jose A.; Hernandez-Avila, Juan E.
Resumo: The site in the midguts of Anopheles pseudopunctipennis where the development of Plasmodium vivax circumsporozoite protein Vk210 phenotype is blocked was investigated, and compared to its development in An. albimanus. Ookinete development was similar in time and numbers within the blood meal bolus of both mosquito species. But, compared to An. pseudopunctipennis, a higher proportion of An. albimanus were infected (P = 0.0001) with higher ookinete (P = 0.0001) and oocyst numbers (P = 0.0001) on their internal and external mid ut surfaces, respectively. Ookinetes were located in the peritrophic matrix (PM), but neither inside epithelial cells nor on the haemocoelic midgut surface by transmission electron microscopy in 24 h p.i.-An. pseudopunctipennis mosquito samples. In contrast, no parasites were detected in the PM of An. albimanus at this time point. These results suggest that P. vivax Vk210 ookinetes cannot escape from and are destroyed within the midgut lumen of An. pseudopunctipennis. (c) 2006 Elsevier Inc. All rights reserved.
Palavras-Chave: Apicomplexa; Malaria; Plasmodium viuax Vk210; Parasite; An. albimanus; An. pseudopunctipennis; Mosquito; Ookinete; Oocyst; Sporozoite; Development; Migration; Circumsporozoite protein; Peritrophic matrix; Deoxyribonucleic acid; Polimerase chain reaction; Monoclonal antibody; Immuno fluorescence assay; Sodium chloride
Imprenta: Experimental Parasitology, v. 115, n. 1, p. 59-67, 2007
Identificador do objeto digital: 10.1016/j.exppara.2006.06.001
Descritores: Aedes aegypti - Cell
Data de publicação: 2007
