Autor(es): Ham, PJ; Yang, HB; Nolan, GP

Resumo: We report the phenotypic selection of two lines of Aedes aegypti, from a filariae susceptible parental stock (Refm). This selection was based upon the level of inducible anti-gram negativeEscherichia coli activity within the haemolymph following E. coli infection. These lines, denoted 'high' and 'low', demonstrated significant differences in anti-E. coli responses throughout. However no difference was observed in the anti-gram positive Micrococcus luteus response following E. coli challenge. F-4, F-6 and F-9 mosquitoes were experimentally infected with Brugia pahangi microfilariae. Reductions of between 53 and 82% in the mean number of larvae completing development in the 'high' compared with the 'low' responding line were observed. Corresponding reductions of between 30 and 50% in the mosquito infection rates also occurred between these lines. These reductions were significant for trials on the F-6 and F-9 generations. We have therefore selected for Ae. aegypti possessing significant refractoriness to filaria infection. Importantly, this 'high' line has not been exposed to selection pressure using filariae development as a phenotypic selective marker.

Palavras-Chave: Insect immunity; Mosquito; Defensins; Peptides

Imprenta: Proceedings of the Royal Society B-Biological Sciences, v. 263, n. 1374, p. 1205-1210, 1996

Identificador do objeto digital: 10.1098/rspb.1996.0177

Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure

Data de publicação: 1996

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