Overexpression of phosphatase and tensin homolog improves fitness and decreases Plasmodium falciparum development in Anopheles stephensi
Autor(es): Hauck, Eric S.; Antonova-Koch, Yevgeniya; Drexler, Anna; Pietri, Jose; Pakpour, Nazzy; Liu, Darin; Blacutt, Jacob; Riehle, Michael A.; Luckhart, Shirley
Resumo: The insulin/insulin-like growth factor signaling (ITS) cascade is highly conserved and regulates diverse physiological processes such as metabolism, lifespan, reproduction and immunity. Transgenic overexpression of Akt, a critical regulator of IIS, was previously shown to shorten mosquito lifespan and increase resistance to the human malaria parasite Plasmodium falciparum. To further understand how IIS controls mosquito physiology and resistance to malaria parasite infection, we overexpressed an inhibitor of IIS, phosphatase and tensin homolog (PTEN), in the Anopheles stephensi midgut. PTEN overexpression inhibited phosphorylation of the ITS protein FOXO, an expected target for PTEN, in the midgut of A. stephensi. Further, PTEN overexpression extended mosquito lifespan and increased resistance to P. falciparum development. The reduction in parasite development did not appear to be due to alterations in an innate immune response, but rather was associated with increased expression of genes regulating autophagy and stem cell maintenance in the midgut and with enhanced midgut barrier integrity. In light of previous success in genetically targeting the ITS pathway to alter mosquito lifespan and malaria parasite transmission, these data confirm that multiple strategies to genetically manipulate US can be leveraged to generate fit, resistant mosquitoes for malaria control. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Palavras-Chave: Phosphatase and tensin homolog (PTEN); Plasmodium falciparum; Mosquito; Insulin/insulin-like growth factor signaling (IIS); Malaria; Anopheles stephensi
Imprenta: Microbes and Infection, v. 15, n. 12, p. 775-787, 2013
Identificador do objeto digital: 10.1016/j.micinf.2013.05.006
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure
Data de publicação: 2013
