Cytogenetic analysis of metaphase chromosomes from pupal testes of four mosquito species using fluorescence in situ hybridization technique (FISH)
Autor(es): Sallam, FAE; Abou El Ela, RG
Resumo: The DNA probes, P1887, P2405, P2056 (being specific tags for Aedes aegypti genes coding for ribosomal RNA) and a centric heterochromatin probe, K20-1A5, were chosen to hybridize the metaphase chromosomes from the testes of four mosquito species, Culex pipiens, Aedes aegypti, Aedes albopictus and Aedes triseriatus. In addition, a single plasmid, P2392, which contained the three probes, P1887, P2405 and P2056, was also used as chromosome landmark in aedine species. Only the Aedes aegypti metaphase chromosome 1-specific tag, P1887, was conserved in Aedes albopictus, Aedes triseriatus, and Culex pipiens metaphase chromosomes. Aedes triseriatus exhibited two gene loci, on chromosomes 1 and 3, coding for ribosomal RNA per haploid genome. When the specific probes for chromosomes 2 and 3, 2405 and 2056, were used in the fluorescence in situ hybridization technique against the metaphase chromosomes the fluorescent signals were not seen in Aedes albopictus, Aedes triseriatus or Culex pipiens. Also the centric heterochromatin probe, K20-1A5, exhibited strong fluorescent signals on chromosomes 1, 2 and 3 of Aedes aegypti. These fluorescent signals were not observed in metaphase chromosomes derived from the other aedine species, indicating that the centromere sequence can vary within the species.
Palavras-Chave: Aedes aegypti; Aedes albopictus; Aedes triseriatus; Culex pipiens; Fluorescence in situ hybridization; Tags; Metaphase chromosomes; Pupal testes
Imprenta: World Journal of Microbiology and Biotechnology, v. 21, n. 4, p. 515-518, 2005
Identificador do objeto digital: 10.1007/s11274-004-2394-y
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - RNA
Data de publicação: 2005
