Autor(es): Wirth, MC; Federici, BA; Walton, WE

Resumo: Bacillus sphaericus is a mosquitocidal bacterium recently developed as a commercial larvicide that is used worldwide to control pestiferous and vector mosquitoes. Whereas B, sphaericus is highly active against larvae of Culex and Anopheles mosquitoes, it is virtually nontoxic to Aedes aegypti, an important vector species, In the present study, we evaluated the capacity of the cytolytic protein Cyt1A from Bacillus thuringiensis subsp. israelensis to enhance the toxicity of B, sphaericus toward A. aegypti. Various combinations of these two materials were evaluated, and all were highly toxic. A ratio of 10:1 of B, sphaericus to Cyt1A was 3,600-fold more toxic to A. aegypti than B, sphaericus alone. Statistical analysis showed this high activity was due to synergism between the Cyt1A toxin and B. sphaericus, These results suggest that Cyt1A could be useful in expanding the host range of B, sphaericus.

Palavras-Chave: Culex - Quinquefasciatus diptera; Mosquitocidal delta - Endotoxin; Pesticidal crystal proteins; Subsp Israelensis; Larvicidal activity; Molecular - Cloning; multiple toxins; Resistance; Larvae; Gene

Imprenta: Applied and Environmental Microbiology, v. 66, n. 3, p. 1093-1097, 2000

Identificador do objeto digital: 10.1128/AEM.66.3.1093-1097.2000

Descritores: Aedes aegypti - Larvicide

Data de publicação: 2000