Culex quinquefasciatus storage proteins
Autor(es): Martins, Larissa A.; Fogaca, Andrea C.; Tania Bijovsky, A.; Carballar-Lejarazu, Rebeca; Marinotti, Osvaldo; Cardoso, Andre F.
Resumo: Insect storage proteins accumulate at high levels during larval development of holometabolous insects. During metamorphosis they are degraded, supplying energy and amino acids for the completion of adult development. The genome of Culex quinquefasciatus contains eleven storage protein-coding genes. Their transcripts are more abundant in larvae than in pupae and in adults. In fact, only four of these genes are transcribed in adults, two of which in blood-fed adult females but not in adult males. Transcripts corresponding to all Cx. quinquefasciatus storage proteins were detected by RTPCR, while mass spectrometric analysis of larval and pupal proteins identified all storage proteins with the exception of one encoded by Cq LSP1.8. Our results indicate that the identified Cx. quinquefasciatus storage protein-coding genes are candidates for identifying regulatory sequences for the development of molecular tools for vector control.
Palavras-Chave: West Nile - Virus; Insecticide resistance; Molecular - Cloning; Aedes aegypti; Fat - Body; Vitellogenin synthesis; Arthropod hemocyanins; Vector competence; Pipiens diptera; Mosquito
Imprenta: Plos One, v. 8, n. 10, 2013
Identificador do objeto digital: 10.1371/journal.pone.0077664
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Protein synthesis
Data de publicação: 2013
