Cross-resistance to pyrethroid and organophosphorus insecticides induced by selection with temephos in Aedes aegypti (Diptera : Culicidae) from Cuba
Autor(es): Rodriguez, MM; Bisset, J; Ruiz, M; Soca, A
Resumo: A sample of Aedes aegypti (L.) from Santiago de Cuba, Cuba, with a high level of temephos resistance (19.58x at the 50% lethal concentration [LC50]),was subjected to temephos selection to evaluate the utility of this organophosphate insecticide for mosquito control. High resistance developed after six generations of selection (200.00x). Little or no cross-resistance was observed to the organophosphates, malathion and fenitrothion, but high cross-resistance was observed for the pyrethroid deltamethrin (337.5x) and the organophosphate fenthion (12.74x). Synergism tests implicated detoxifying esterases in temephos and fenthion resistance and deltamethrin resistance was associated with a cytochrome P450 monooxygenasa. Biochemical tests, polyacrylamide gel electrophoresis (PAGE), and inhibition studies confirmed the presence of elevated esterase activity associated with temephos resistance. Pyrethroid resistance could be associated with a glutathione-S-transferase mechanism but not the esterases. The cross-resistance to deltamethrin from temephos selection could limit the use of both insecticides for Ae. aegypti control.
Palavras-Chave: Aedes aegypti; Resistance; Organophosphate; Pyrethroideste; Raseglutathione-S-transferase
Imprenta: Journal of Medical Entomology, v. 39, n. 6, p. 882-888, 2002
Identificador do objeto digital: 10.1603/0022-2585-39.6.882
Descritores: Aedes aegypti - Public health
Data de publicação: 2002
