Competitive suppression between chikungunya and dengue virus In Aedes albopictus C6/36 cell line
Autor(es): Potiwat, Rutcharin; Komalamisra, Narumon; Thavara, Usavadee; Tawatsin, Apiwat; Siriyasatien, Padet
Resumo: Aedes albopictus C6136 cell line was used to evaluate dengue virus sero-type-3 (DENV-3) and chikungunya virus (CHIKV) co-infection. Virus infection was determined using a one-step duplex reverse transcriptase polymerase chain reaction (D- RT-PCR). D-RT-PCR was positive for both viruses when equal multiplicity of infection (MOI) was utilized. Co-infection with different titers between a higher CHIKV titer (MOI = 1.0) than DENV-3 titer (MOI = 0.1) showed similar results with that of equal titer. However, co-infection with a lower CHIKV titer (MOI = 0.1) than DENV-3 titer (MOI = 1.0) revealed positive results for only dengue virus infection, suggesting DENV competitive suppression of CHIKV. This competitive suppression occurred in mixed-infection samples but not in individually double infection (super infection) samples which produced both dengue and chikungunya virus progenies. Both virus replications depend on the amount of virus titer rather than serial infection. These findings have provided information regarding pathogen-pathogen interaction in host cell, which could be used to predict outbreaks, and to develop virus detection and vector control.
Palavras-Chave: Chikungunya virus; Dengue virus; Aedes albopictus C6/36 cell line; Competitive suppression
Imprenta: Southeast Asian Journal of Tropical Medicine and Public Health, v. 42, n. 6, p. 1388-1394, 2011
Descritores: Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Chikungunya Fever
Data de publicação: 2011
