Comparison of the activities of Bacillus thuringiensis mosquitocidal crystal proteins on mosquito cell lines (Diptera, Culicidae), using two colorimetric methods
Autor(es): Laurent, P; Charles, JF; Dumanoir, VC; Lecadet, MM
Resumo: Two colorimetric methods were compared for assaying the cell toxicity of soluble crystal proteins from five mosquitocidal serovarieties of Bacillus thuringiensis: israelensis, jegathesan, medellin, darmstadiensis and fukuokaensis. In mosquitoes cell lines from Culex quinquefasciatus, Aedes aegypti and Anopheles gambiae, all the crystal proteins were equally toxic. Both colorimetric methods MTT (tetrazolium salt) and Alamar Blue gave similar results, but the Alamar Blue method was simpler, faster, cheaper, and could be used to take readings from the same cell population at various time points. The most active crystal proteins were those of B. thuringiensis israelensis, followed by those of B. thuringiensis jegathesan, and B. thuringiensis medellin, which were much less active.
Palavras-Chave: Alamar Blue; Bacillus thuringiensis; Crystal toxin; Mosquito cell; MTT
Imprenta: World Journal of Microbiology and Biotechnology, v. 16, n. 1, p. 37-41, 2000
Identificador do objeto digital: 10.1023/A:1008904101774
Descritores: Aedes aegypti - Cell
Data de publicação: 2000
