Autor(es): Lee, Dae-Weon

Resumo: Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT7 receptor of Aedes aegypti, the Aedes 5-HT7 receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-K1 cells. The expression of the Aedes 5-HT7 receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT7 receptor is much more effective in mammalian CHO-K1 cells and that the Tr-CHO cell line expressing Aedes 5-HT7 receptor can be used for synthetic agonist or antagonist candidate screening.

Palavras-Chave: Serotonin receptor, Aedes aegypti, cAMP, Serotonin, Immunocytochemistry

Imprenta: Korean Journal of Applied Entomology, v. 43, n. 2, p. 155-162, 2004

Descritores: Aedes aegypti - Molecular screening

Data de publicação: 2004

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