Cognate chitinoproteins are detected during Drosophila melanogaster development and in cell cultures from different insect species
Autor(es): Kramerov, AA; Rozovsky, YM; Baikova, NA; Gvozdev, VA
Resumo: Chitinase-sensitive glycoproteins (chitinoproteins) were detected by [H-3]D-glucosamine incorporation and polyclonal antibodies against D. melanogaster chitinoprotein (Kramerov et al., Insect Biochem., 16, 417-432, 1986) in established cell cultures from different insect species (D. hydei, D. virilis, mosquito Aedes aegypti and silkworm Bombyx mori). It was shown that reversible oxidation of disulfide bonds results in two interconvertible monomeric and dimeric forms of D. melanogaster chitinoprotein with apparent molecular masses of 100,000 and 200,000, respectively. Similar glycoproteins with molecular masses corresponding to monomeric (80,000) and dimeric (160,000) forms were detected in the D. hydei, D. virilis and A. aegypti cells. The cognate chitinoprotein with a molecular mass of 100,000 was revealed in cultured cells of B. mori. These chitinoproteins were localized by immunostaining within cytoplasmic granules in all of the cultured cells tested.Both forms of the chitinoprotein with molecular masses of 80,000 and 160,000 were continuously detected throughout the development of D. melanogaster, with the pupal stage being the most abundant.
Palavras-Chave: Insect cultured cells; Chitinase - Sensitive glycoproteins; Chitinoproteins; Polyclonal antibodies; Western blotting; Immunostaining; Drosophila development
Imprenta: Insect Biochemistry, v. 20, n. 8, p. 769-775, 1990
Identificador do objeto digital: 10.1016/0020-1790(90)90094-B
Descritores: Aedes aegypti - Protein synthesis
Data de publicação: 1990
