Cloning and sequence analysis of para sodium channel cDNA fragment from silkworm, Bombyx mori
Autor(es): He Lin; Liu Li-Hua; Wang Yang
Resumo: Previous studies have revealed that a point mutation of a target gene is related to insecticide resistance to pyrethroids. The pare sodium channel in the insect central nervous system is the target of pyrethroid insecticides. We used the RT-PCR method to clone the para sodium ion channel in the silkworm, Bombyx mori L. ( GenBank No. EF521818). The full length of this cDNA fragment is 4 882 base pairs and its partial ORF is 3 986 bp translated into 1 328 amino acids. BLAST analysis demonstrated that the cloned cDNA fragment is virtually identical to the para sodium channel alpha subunit gene amplified from other insects. Amino acid homology of the cloned fragment with pare sodium channel alpha subunit genes from Heliothis virescens Fabricius, Aedes aegypti L., Blattella germanica L., Drosophila melanogaster Meigen and Musca domestica L. was 95%, 82%, 80%, 79% and 77% respectively.
Palavras-Chave: Silkworm; Para - Sodium channel; Gene clone; Sequence analysis
Imprenta: Chinese Journal of Applied Entomology, v. 48, n. 3, p. 596-604, 2011
Descritores: Aedes aegypti - RT-PCR
Data de publicação: 2011
