Autor(es): Tan, Wenbin; Wang, Xiao; Cheng, Peng; Liu, Lijuan; Wang, Haifang; Gong, Maoqing; Quan, Xin; Gao, Honggang; Zhu, Changliang

Resumo: The complete sequence of transferrin has been cloned from cypermethrin-resistant strain of Culex pipiens pallens(Cr-C strain). Quantitative reverse transcription PCR analysis indicated that the transferrin transcription level was 12.6 times higher in Cr-C strain than in susceptible strain at fourth instar larvae. The transferrin expression was also found to be consistently higher throughout the life cycle of Cr-C strain. A protein of predicted size 90.8 kDa has been detected by Western blotting in transferrin-transfected mosquito C6/36 cells. These transferrin-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by methyl tritiated thymidine(H-3-TdR) incorporation. These results indicate that transferrin is expressed at higher levels in Cr-C strain and may confer some insecticide resistance in C. pipiens pallens.

Palavras-Chave: TREATED MOSQUITO NETS; AEDES-AEGYPTI; LARVICIDAL ACTIVITY; DELTAMETHRIN RESISTANCE; GENOME SCAN; INSECTICIDE; EXPRESSION; EVOLUTION; INFECTION; SEQUENCE

Imprenta: Parasitology Research, v. 110, n. 2, p. 939-959, 2012

Identificador do Objeto Digital: 10.1007/s00436-011-2580-4

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome

Data de Publicação: 2012