Cloning and expression pattern of 14-3-3 epsilon from Culex pipiens
Autor(es): Kim, Dong Hyun; Jo, Yong Hun; Han, Yeon Soo; Kang, Sang Sun; Chun, Jaesun
Resumo: 14-3-3 proteins play various roles in eukaryotes, such as signal transduction, cell cycle regulation, apoptosis, stress response and cytoskeleton organization. We are interested in apoptosis induced by mosquito-virus interactions using Culex pipiens as an experimental model. To this end, we cloned a partial fragment (702 bp) of 14-3-3 epsilon, one of the key proteins known to be involved in apoptosis, from Cx. pipiens. The deduced amino acid sequence (234 residues) had high homology to 14-3-3 epsilon homologs from various organisms, such as Culex quinquefasciatus (99%), Anopheles gambiae (98%), Aedes aegypti (96%), Drosophila melanogaster (93%), Apis mellifera (91%) and Bombyx mori (89%). Developmental reverse transcription (RT)-polymerase chain reaction (PCR) analysis showed that Cp14-3-3 epsilon mRNA is expressed at very high levels in the egg, early pupa, and adult male and female, although it was expressed in other developmental stages, such as first and fourth instar larvae. Analysis of RT-PCR for tissue specificity showed that Cp14-3-3 epsilon is abundant in the abdomen and ovary, and it was also detectable in all other tissues, such as thorax, midgut and Malpighian tubule. Actinomycin-D, an apoptosis-inducing chemical, induces Cp14-3-3 epsilon mRNA expression. Furthermore, poly I : C (RNA virus analog) induces Cp14-3-3 epsilon mRNA 3 h after injection. Thus, although further investigation is necessary for elucidating the biological significance of the induced 14-3-3 epsilon, our data suggest that Cp14-3-3 epsilon is involved in cell survival and signal transduction in Cx. pipiens.
Imprenta: Entomological Research, v. 39, n. 3, p. 192-195, 2009
Identificador do objeto digital: 10.1111/j.1748-5967.2009.00223.x
Descritores: Aedes aegypti - RNA ; Aedes aegypti - RT-PCR
Data de publicação: 2009
