Autor(es): Barloy, F; Delecluse, A; Nicolas, L; Lecadet, MM

Resumo: A gene (cbm71) encoding a 71,128-Da mosquitocidal protein (Cbm71) was obtained by screening a size-fractionated XbaI digest of total genomic DNA from Clostridium bifermentans subsp. malaysia CH18 with two gene-specific oligonucleotide probes. The sequence of the Cbm71 protein, as deduced from the sequence of cbm71, corresponds to that of the 66-kDa protein previously described as one of the mosquitocidal components of C. bifermentans subsp. malaysia. Cbm71 shows limited similarities with Bacillus thuringiensis delta-endotoxins, especially in the four first conserved blocks. However, Cbm71 was not immunologically related to any of the Cry toxins and thus belongs to a novel class of mosquitocidal protein. The cbm71 gene was expressed in a nontoxic strain of B. thuringiensis, and Cbm71 was produced during sporulation and secreted to the supernatant of culture. Trichloroacetic-precipitated supernatant preparations were toxic for mosquito larvae of the species Aedes aegypti, Culex pipiens, and Anopheles stephensi.

Palavras-Chave: Serovar malaysia; Escherichia -Coli; Transformation; Israelensis; Bacterium; Hemolysin; Secretion; Cleavage

Imprenta: Journal of Bacteriology, v. 178, n. 11, p. 3099-3105, 1996

Identificador do objeto digital: 10.1128/jb.178.11.3099-3105

Descritores: Aedes aegypti - DNA

Data de publicação: 1996

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