Autor(es): Marchenko, ND; Marchenko, GN; Ganushkina, LO; Azizbekyan, RR

Resumo: The cryIVB gene from a new isolate of Bacillus thuringiensis var israelensis was cloned and sequenced. Two nucleotide replacements resulted in changing Asp(385)-Thr(386) to Glu(385)-Ser(386) were found in comparison with the previously sequenced cryIVB gene. Two genetic constructions were designed for expression of cryIVB in the obligate methylotroph Methylobacillus flagellatum, In the first construction, cryIVB was cloned under the strong inducible lac promoter and contained original ribosome binding site and 150 bp of 5' transcribed but untranslated region. In the second construct, the first five codons of the lacZ gene were fused to the second codon of the cryIVB gene. Both E. coli and M. flagellatum harboring both constructs were toxic to insect larvae of Anopheles stephensi and Aedes aegypti. However, the toxicity of the methylotroph was about 450 times less. This study is the first attempt to use methylotrophs as an insecticidal endotoxin producer.

Palavras-Chave: B. thuringiensis; Delta - Endotoxin; CryIVB; Mosquitocidal toxin; Methylotroph

Imprenta: Journal of Industrial Microbiology & Biotechnology, v. 24, n. 1, p. 14-18, 2000

Identificador do objeto digital: 10.1038/sj.jim.2900754

Descritores: Aedes aegypti - Biosynthesis

Data de publicação: 2000

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