Chitin is only a minor component of the peritrophic matrix from larvae of Lucilia cuprina
Autor(es): Tellam, RL; Eisemann, C
Resumo: The gut of most insects is lined with a peritrophic matrix that facilitates the digestive process and protects insects from invasion by micro-organisms and parasites. It is widely accepted that the matrix is composed of chitin, proteins and proteoglycans. Here we critically re-examine the chitin content of the typical type 2 peritrophic matrix from the larvae of the fly Lucilia cuprina using a range of techniques. Many of the histochemical and biochemical techniques indicate the presence of chitin, although they are often adversely influenced by the presence of highly glycosylated proteins, a principal component of the matrix. The alkali-stable fraction, which is used as an indicator of the maximum chitin content in a biological sample, is only 7.2% of the weight of the matrix. Larvae fed on the potent chitin synthase inhibitor polyoxin D or the chitin-binding agent Calcofluor White, showed strong concentration-dependent inhibition of larval weight and survival but no discernible effects on the matrix structure. A bacterial endochitinase fed to larvae had no effect on larval growth and no observable effect in vitro on the structure of isolated peritrophic matrix. RT-PCR did not detect a chitin synthase mRNA in cardia, the tissue from which PM originates. It is concluded that chitin is a minor structural component of the type 2 peritrophic matrix of this insect. (C) 2000 Elsevier Science Ltd. All rights reserved.
Palavras-Chave: Peritrophic matrix; Peritrophic membrane; Peritrophin; Lucilia cuprina; Chitin
Imprenta: Insect Biochemistry and Molecular Biology, v. 30, n. 12, p. 1189-1201, 2000
Identificador do objeto digital: 10.1016/S0965-1748(00)00097-7
Descritores: Aedes aegypti - RT-PCR
Data de publicação: 2000
