Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae
Autor(es): Garczynski, SF; Crim, JW; Brown, MR
Resumo: The genome of Anopheles gambiae contains sequences encoding a neuropeptide F (Ang-NPF) and NPF receptor (Ang-NPFR) related to the neuropeptide Y signaling family. cDNAs for each were cloned and sequenced. Ang-NPFR was stably expressed for radioligand binding analysis. Ang-NPF exhibited high affinity (IC50 similar to 3 nM) membrane binding; NPFs from Aedes aegypti (Aea-NPF) and Drosophila melanogaster (Drm-NPF) were less potent, with the rank order Ang-NPF > Aea-NPF > Drm-NPF > Drm-NPF8-36. RT-PCR analysis revealed Ang-NPF and Ang-NPFR transcripts in all life stages. Ang-NPF and Ang-NPFR may be strategically positioned for signaling in relation to nutritional status in the African malaria mosquito. (C) 2004 Elsevier Inc. All rights reserved.
Palavras-Chave: Insect; Diptera; Anopheles; Malaria; G protein - Coupled receptor; Neuropeptide F; Neuropeptide Y
Imprenta: Peptides, v. 26, n. 1, p. 99-107, 2005
Identificador do objeto digital: 10.1016/j.peptides.2004.07.014
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - RT-PCR
Data de publicação: 2005
