Characterization of an infectious clone of the wild-type yellow fever virus Asibi strain that is able to infect and disseminate in mosquitoes
Autor(es): McElroy, KL; Tsetsarkin, KA; Vanlandingham, DL; Higgs, S
Resumo: Infectious clone technology provides an opportunity to study the molecular basis of arthropod-virus interactions in detail. This study describes the development of an infectious clone of the prototype yellow fever virus Asibi strain (YFV-As) with the purpose of identifying sequences or domains that influence infection dynamics in the mosquito vector. The full-length cDNA of YFV-As virus was produced from RT-PCR products of parental viral RNA. These were cloned into a low-copy-number plasmid previously used to develop the YFV-17D infectious clone (pACNR/FLYF-17D). Virus recovered from the infectious clone exhibited biological characteristics similar to those of the parental YFV-As, including replication kinetics, reactivity to flavivirus cross-reactive and YFV-specific antibodies and infection and dissemination rates in Aedes aegypti, the principal mosquito vector of YFV. These data provide the basis for future studies with chimeric Asibi/17D viruses to identify the determinants of vaccine attenuation in the vector.
Palavras-Chave: Borne Encephalitis - Virus; CDNA - Clone; In vitro; Flavivirus; RNA; Attenuation; Mice; 17d
Imprenta: Journal of General Virology, v. 86, p. 1747-1751, 2005
Identificador do objeto digital: 10.1099/vir.0.80746-0
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - RT-PCR ; Aedes aegypti - Virus ; Aedes aegypti - Public health
Data de publicação: 2005
