Characterization and expression of cry4Cb1 and cry30Ga1 from Bacillus thuringiensis strain HS18-1
Autor(es): Zhu, Jun; Zheng, Aiping; Wang, Shiquang; Liu, Huainian; Li, Ping
Resumo: We characterized a novel Bacillus thuringiensis isolate native to China (HS18-1) that shows a spherical crystal harboring two major proteins of about 70 and 130 kDa, and contains three novel cry genes (cry4Cb1, cry30Ga1, cry54-type). Furthermore, the cry4Cb1 and cry30Ga1 genes were expressed in Escherichia coli BL21 (DE3): pLysS. Insecticidal activity tests showed that the cry4Cb1 protein exhibited larvicidal activity against Aedes aegypti (Diptera) and the cry30Ga1 protein was toxic to both A. aegypti and P. xylostella (Lepidoptera). (C) 2009 Elsevier Inc. All rights reserved.
Palavras-Chave: B. thuringiensis; Cry4Cb1; Cry30Ga1; Insecticidal activity
Imprenta: Journal of Invertebrate Pathology, v. 103, n. 3, p. 200-202, 2010
Identificador do objeto digital: 10.1016/j.jip.2009.12.004
Descritores: Aedes aegypti - Larvicide
Data de publicação: 2010
