Characterization and expression of a novel holotype insecticidal crystal protein gene from native Bacillus thuringiensis BM59-2
Autor(es): Zheng, Aiping; Zhu, Jun; Wang, Lingxia; Li, Shuangcheng; Deng, Qiming; Wang, Shiquan; Tan, Furong; Yu, Xiumei; Guan, Peng; Liang, Hongxia; Li, Ping
Resumo: We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 mu g/mL (95% confidence: 0.740- 3.499 mu g/mL).
Palavras-Chave: Bacillus thuringiensis; Cry52Ba1; Characterization - Expression; Insecticidal activity
Imprenta: Canadian Journal of Microbiology, v. 56, n. 2, p. 156-161, 2010
Identificador do objeto digital: 10.1139/W09-120
Descritores: Aedes aegypti - Proteins
Data de publicação: 2010
