Black fly salivary gland extract inhibits proliferation and induces apoptosis in murine splenocytes
Autor(es): Tsujimoto, H.; Gray, E. W.; Champagne, D. E.
Resumo: Black flies are known to be vectors of pathogens including Onchocerca volvulus, which causes human onchocerciasis, and Vesicular Stomatitis Virus. Their salivary secretion has been shown to contain a complex cocktail of anti-haemostatic factors and immunomodulatory activities, which may contribute to efficient transmission of the pathogens. Black fly salivary gland extract (SGE) inhibits mitogen-stimulated mouse splenocyte proliferation, including proliferation of both CD4+ and CD8+ T cells. The factor responsible for the inhibition was determined to be a protein (or protein complex) of a size larger than 50 kDa. Moreover, exposure to SGE results in activation of caspase 3 and characteristic morphological changes in CD4+ and CD8+ T cells, suggesting that induction of apoptosis could, at least in part, be responsible for this inhibition.
Palavras-Chave: Apoptosis; Black fly; Saliva; T lymphocyte
Imprenta: Parasite Immunology, v. 32, n. 4, p. 275-284, 2010
Identificador do objeto digital: 10.1111/j.1365-3024.2009.01186.x
Descritores: Aedes aegypti - Cell ; Aedes aegypti - T lymphocytes
Data de publicação: 2010
